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Rat PDGF-CC PicoKine ELISA Kit

分類:

ELISA試劑盒 - Rat Elisa Kit

說明書:

EK1855

2280元/96T  

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---元*1

選擇數量:

- +

Product Brief

  • 產品概況

    產品名稱 Rat PDGF-CC PicoKine ELISA Kit
    產品描述 For quantitative detection of rat PDGF-CC in cell culture supernates, serum and plasma (heparin, EDTA).
    驗證種屬
    免疫原 標準品表達系統:E.coli, A235-G345
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    規格 96wells/kit, with removable strips.

    產品性質

    敏感性 <10pg/ml
    *敏感性/最低檢測濃度計算方式:敏感性等于20個空白對照孔的平均O.D.值加上2個標準差后對應的濃度。樣品的O.D.讀數只有在高過敏感性指標的時候才具有統計學顯著性。
    檢測范圍 15.6pg/ml-1000pg/ml *檢測范圍不代表敏感性。檢測范圍是以常見樣品的指標表達量為參照,在博士德內部質檢過程中使用的標準曲線區間。如果用戶需要,可以加大標準曲線的初始稀釋度來讓試劑盒檢測更低的范圍。更多實驗小貼士參見博士德的技術資料中心
    樣品類型 cell culture supernates, serum and plasma (heparin, EDTA). *對于上述未提及的樣品類型,在一般情況下博士德的ELISA試劑盒可以檢測。由于未檢測的樣品可能出現表達量過低的情況,建議先進行小規模實驗驗證可行性。
    包被抗體 a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
    檢測抗體 a biotinylated polyclonal antibody from goat
    一般表達濃度 以下文章提供了Pdgfc的表達量的信息:
    保存條件 4℃六個月,-20℃一年。避免不必要的重復溶解、凍結(加冰郵寄)

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1855-CAP96-well plate precoated with anti- rat PDGF-CC antibody1
    EK1855-STLyophilized recombinant rat PDGF-CC standard10ng/tube×2
    EK1855-DABiotinylated anti- rat PDGF-CC antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    指標相關信息 (來源: Uniprot.org)

    小貼士:你可以利用tissue specificity信息來幫助設計陽性和陰性對照。對此有疑問請聯系我們技術服務部門。
    基因名 Pdgfc
    蛋白名 Platelet-derived growth factor C
    基因全名 platelet derived growth factor C
    Uniprot ID Pdgfc: Q9EQX6
    指標別名 Platelet-derived growth factor C| PDGF-C| Fallotein| Spinal cord-derived growth factor| SCDGF| VEGF-E| Platelet-derived growth factor C, latent form| PDGFC latent form| Platelet-derived growth factor C, receptor-binding form| PDGFC receptor-binding form| Pdgfc| Scdgf

    platelet derived growth factor C指標簡介

    Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. It is mapped to 2q33-q34. Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation.

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision

    Rat PDGF-CC PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 15-20)
    Concentration(pg/ml)015.631.262.51252505001000
    O.D.0.0320.0850.1430.2480.4230.8161.3592.231
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

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