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Rat Matrilin-4 PicoKine ELISA Kit

分類:

ELISA試劑盒 - Rat Elisa Kit

說明書:

EK2031

2280元/96T  

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---元*1

選擇數量:

- +

Product Brief

  • 產品概況

    產品名稱Rat Matrilin-4 PicoKine ELISA Kit
    產品描述For quantitative detection of rat Matrilin-4 in cell culture supernates, serum and plasma (heparin, EDTA).
    驗證種屬Rat
    免疫原標準品表達系統:NSO, Q22-K624
    交叉反應There is no detectable cross-reactivity with other relevant proteins.
    規格96wells/kit, with removable strips.

    產品性質

    敏感性<50pg/ml
    *敏感性/最低檢測濃度計算方式:敏感性等于20個空白對照孔的平均O.D.值加上2個標準差后對應的濃度。樣品的O.D.讀數只有在高過敏感性指標的時候才具有統計學顯著性。
    檢測范圍93.7pg/ml-6000pg/ml*檢測范圍不代表敏感性。檢測范圍是以常見樣品的指標表達量為參照,在博士德內部質檢過程中使用的標準曲線區間。如果用戶需要,可以加大標準曲線的初始稀釋度來讓試劑盒檢測更低的范圍。更多實驗小貼士參見博士德的技術資料中心
    樣品類型cell culture supernates, serum and plasma (heparin, EDTA). *對于上述未提及的樣品類型,在一般情況下博士德的ELISA試劑盒可以檢測。由于未檢測的樣品可能出現表達量過低的情況,建議先進行小規模實驗驗證可行性。
    包被抗體a monoclonal antibody from mouse
    檢測抗體a biotinylated polyclonal antibody from goat
    一般表達濃度以下文章提供了Matn4的表達量的信息:
      保存條件4℃六個月,-20℃一年。避免不必要的重復溶解、凍結(加冰郵寄)

      試劑盒內容

      SKU名稱數量
      Catalog numberDescriptionQuantity
      EK2031-CAP96-well plate precoated with anti- rat Matrilin-4 antibody1
      EK2031-STLyophilized recombinant rat Matrilin-4 standard10ng/tube×2
      EK2031-DABiotinylated anti- rat Matrilin-4 antibody130μl(dilution 1:100)
      AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
      AR1106-1Sample diluent buffer30 ml
      AR1106-2Antibody diluent buffer12ml
      AR1106-3ABC diluent buffer12ml
      AR1104TMB color developing agent10ml
      AR1105TMB stop solution10ml
      PLA-SEAAdhesive cover4
      *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

      需要用戶自己提供的器材和試劑

      • 1.96孔讀板儀器。
      • 2.自動洗板機.
      • 3.移液器和槍頭。建議使用多通道移液器/排槍。
      • 4.Eppendorf 管。
      • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
        • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
        • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      *樣品準備的相關試劑也不含在試劑盒中。

      指標相關信息 (來源: Uniprot.org)

      小貼士:你可以利用tissue specificity信息來幫助設計陽性和陰性對照。對此有疑問請聯系我們技術服務部門。
      基因名Matn4
      蛋白名Matrilin 4
      基因全名matrilin 4
      Uniprot IDMatn4: D3ZMS3
      指標別名Matrilin 4; Matn4; Matn4_predicted; rCG_32351

      matrilin 4指標簡介

      Matrilin-4 is a 73 kDa secreted glycoprotein that is a member of the matrilin family of the von Willebrand Factor-A (vWA) domain-containing superfamily. This gene is mapped to 3q42. Matrilins are modular extracellular matrix proteins that serve as adaptors and linkers for other matrix proteins. Matrilin-4, like Matrilin?2, has a broad distribution in both cartilage and in loose connective tissue such as dermis, lung and kidney, while Matrilins 1 and 3 are limited to cartilage. Matrilin-4 is present in nervous tissue and is abundant in the brain. Mature mouse Matrilin-4 shares 98%, 90%, 89% and 66% amino acid (aa) identity with rat, human, canine and chicken Matrilin-4, respectively. The 624 aa mouse Matrilin-4 contains a 22 aa signal sequence, two potential glycosylation sites, and four cysteine-rich EGF-like domains placed between two vWA domains. A short isoform lacks the N-terminal vWA domain (aa 28?217). A C-terminal alpha -helix/coiled-coil region (aa 590?623) by which multimers are formed is often proteolytically removed so that Matrilin-4 is found as a mixture of monomers with homo- or hetero- dimers and trimers. Matrilin-4 forms multimers with Matrilins 1 and 2 but not with Matrilin-3. The N-terminal vWA domains of Matrilins associate with collagen IV microfibrils via the proteoglycans biglycan and decorin, linking the fibrils with other matrix constituents aggrecan and collagen II. Matrilins also show calcium-dependent binding to the cartilage oligomeric matrix protein (COMP); this interaction is of high affinity for oligomeric Matrilin-4 and somewhat lower affinity for monomeric Matrilin-4. Functions and distributions of Matrilins overlap enough so that knockouts of Matrilins 1, 2 and 3 lack obvious phenotypes.

      批內差/批間差

      Intra-Assay PrecisionInter-Assay Precision

      Rat Matrilin-4 PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

      (TMB 孵育條件: 37°C, 15-20)
      Concentration(pg/ml)093.7187.5375750150030006000
      O.D.0.0350.1370.2280.4200.7741.1371.7622.147
      *典型數據僅供參考。用戶需要自己建立標準曲線。

    Instructions

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