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Rat ANGPTL2 PicoKine ELISA Kit

分類:

ELISA試劑盒 - Rat Elisa Kit

說明書:

EK2041

2280元/96T  

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---元*1

選擇數量:

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Product Brief

  • 產品概況

    產品名稱Rat ANGPTL2 PicoKine ELISA Kit
    產品描述For quantitative detection of rat ANGPTL2 in cell culture supernates, serum and plasma (heparin, EDTA).
    驗證種屬Rat
    免疫原標準品表達系統:NSO, A20-H493
    交叉反應There is no detectable cross-reactivity with other relevant proteins.
    規格96wells/kit, with removable strips.

    產品性質

    敏感性<10pg/ml
    *敏感性/最低檢測濃度計算方式:敏感性等于20個空白對照孔的平均O.D.值加上2個標準差后對應的濃度。樣品的O.D.讀數只有在高過敏感性指標的時候才具有統計學顯著性。
    檢測范圍31.2pg/ml-2000pg/ml*檢測范圍不代表敏感性。檢測范圍是以常見樣品的指標表達量為參照,在博士德內部質檢過程中使用的標準曲線區間。如果用戶需要,可以加大標準曲線的初始稀釋度來讓試劑盒檢測更低的范圍。更多實驗小貼士參見博士德的技術資料中心
    樣品類型cell culture supernates, serum and plasma (heparin, EDTA). *對于上述未提及的樣品類型,在一般情況下博士德的ELISA試劑盒可以檢測。由于未檢測的樣品可能出現表達量過低的情況,建議先進行小規模實驗驗證可行性。
    包被抗體a monoclonal antibody from mouse
    檢測抗體a biotinylated polyclonal antibody from goat
    一般表達濃度以下文章提供了Angptl2的表達量的信息:
      保存條件4℃六個月,-20℃一年。避免不必要的重復溶解、凍結(加冰郵寄)

      試劑盒內容

      SKU名稱數量
      Catalog numberDescriptionQuantity
      EK2041-CAP96-well plate precoated with anti- rat ANGPTL2 antibody1
      EK2041-STLyophilized recombinant rat ANGPTL2 standard10ng/tube×2
      EK2041-DABiotinylated anti- rat ANGPTL2 antibody130μl(dilution 1:100)
      AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
      AR1106-1Sample diluent buffer30 ml
      AR1106-2Antibody diluent buffer12ml
      AR1106-3ABC diluent buffer12ml
      AR1104TMB color developing agent10ml
      AR1105TMB stop solution10ml
      PLA-SEAAdhesive cover4
      *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

      需要用戶自己提供的器材和試劑

      • 1.96孔讀板儀器。
      • 2.自動洗板機.
      • 3.移液器和槍頭。建議使用多通道移液器/排槍。
      • 4.Eppendorf 管。
      • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
        • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
        • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      *樣品準備的相關試劑也不含在試劑盒中。

      指標相關信息 (來源: Uniprot.org)

      小貼士:你可以利用tissue specificity信息來幫助設計陽性和陰性對照。對此有疑問請聯系我們技術服務部門。
      基因名Angptl2
      蛋白名Angiopoietin-like 2
      基因全名angiopoietin-like 2
      Uniprot IDAngptl2: G3V862
      指標別名Angiopoietin-like 2; Angptl2; rCG_45808

      angiopoietin-like 2指標簡介

      Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 3p11. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.

      批內差/批間差

      Intra-Assay PrecisionInter-Assay Precision

      Rat ANGPTL2 PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

      (TMB 孵育條件: 37°C, 15-20)
      Concentration(pg/ml)031.262.512525050010002000
      O.D.0.0680.1100.1520.2280.3760.7561.1792.026
      *典型數據僅供參考。用戶需要自己建立標準曲線。

    Instructions

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